Nuclei Need Nurturing, Too
Laminar Wash™ benefits of automation and gentle cell-based sample preparation can also be extended to nuclei Analytical methods on individual cells provide an enormous amount of useful information. But not all tissue types lend themselves to preparing single cell suspensions necessary for flow cytometry or single-cell ‘omics analysis. Homogenization and dissociation of interconnected tissues such […]
Towards standardizing flow cytometry sample preparation protocols without centrifugation
Now Watchinghttps://youtu.be/NTTfiQiqhqE In this sponsored webinar by the flowcytometryUK society webinar, renowned flow cytometrist Derek Davies, Ph.D. of the Francis Crick Institute hosted a discussion featuring Curiox Applications Scientist Noma Ngwenya and Charles River Laboratories Principal Scientist III and Flow Cytometry Core Director Christoph Eberle, Ph.D. They explored how Laminar Wash™ technology enhances sample preparation for flow cytometry by […]
A Missing Sample Prep Tool for Single-Cell Biology is Found
Gentle and semi-automated Laminar Wash™ technology removes background, debris, doublets, and dead time, extending single-cell ‘omics budgets. Single-cell ‘omics technologies are achieving a new paradigm in biology. As Eadweard Muybridge applied photography to settle the “unsupported transit” controversy surrounding the 19th century galloping horse, immunologists are utilizing single-cell transcriptomics (scRNA) to tease apart the heterogeneity […]
Automated flow cytometry staining protocol video
Now Watching This video demonstrates the AUTO1000 accomplishing an automated standard cell staining protocol to prepare samples for analysis by flow cytometry. The video is set at 40X speed. Notice how fast the Laminar Wash step is executed, relative to the “rate limiting steps” of automatic pipetting, as well as how clear those wells are after washing! Curiox’s […]
Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways
READ FRONTIERS IN IMMUNOLOGY Stewart A., Ng JC-F, Wallis G, et al. Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways. Front. Immunol. 2021;12:602539. Subscribe to our newsletter Get updates about upcoming webinar technical application information and product announcements when you subscribe to the Curiox newsletter.
How to wash away barriers to therapeutic cancer vaccine development
Developing neoantigen-based cancer vaccines necessitates comprehensive cellular characterization for iterative therapeutic improvement, which Laminar Wash™ systems may facilitate at multiple steps. Vaccines are probably the second-most effective advancement in the annals of human medicine, after sanitation. Most of us recognize the value of vaccines in infectious disease. Yet, swept under the rug amongst the COVID-19 global pandemic is another form of immune-boosting injection combating a terrible […]
Centrifuge-Less Automation of Suspension Cell Sample Preparation
Now Watchinghttps://www.youtube.com/watch?v=wkxcKl8DNMc In our webinar from February 24, 2021, Curiox Applications Scientist Ann Wang and Peak Analysis and Automation (PAA) Technical Director Dr. Malcolm Crook discussed how Laminar Wash™ technology can readily automate sample preparation, sans centrifuge. Ann starts things off, introducing Laminar Wash technology and Curiox solutions for automated cell prep. She also addressed questions in the Q&A with data. Highlights include: […]
Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
Now Watching Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries Elijah W. Chen*1, Chyan Ying Ke*2,3, Joanna Brzostek*1, Nicholas R. J. Gascoigne1, Vasily Rybakin1,41Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Singapore Immunology Network, A*STAR, 3Curiox Biosystems, 4Department of Immunobiology, Rega Institute for Medical Research, Katholieke Universiteit (KU) Leuven * These authors […]
Automating cell prep with Laminar Wash–SLAS 2021
Now Watchinghttps://www.youtube.com/watch?v=bisHv1x1lkE Description Laminar Wash technology offers a centrifuge-less sample preparation method that can be readily automated. Our presentation at the Society for Laboratory Automation and Screening (SLAS) 2021 conference, Curiox Applications Scientist Ann Wang and Sales Manager Dr. Darren Warnick discussed cell prep automation. In the first part of the talk, Dr. Darren Warnick […]
Developing a 43 color panel on Cytek Aurora using Laminar Wash for Sample Prep
The advent of modern flow cytometers and flow cytometric methods has accelerated researchers’ ability to enhance the resolution of samples analyzed. Within a span of 20 years, flow cytometry panels have ballooned from a handful of channels to well over 40. The theoretical limit of flow panels now lies not in the available fluorophores or […]