Category: Flow Cytometry

Developing a 43 color panel on Cytek Aurora using Laminar Wash for Sample Prep

The advent of modern flow cytometers and flow cytometric methods has accelerated researchers’ ability to enhance the resolution of samples analyzed. Within a span of 20 years, flow cytometry panels have ballooned from a handful of channels to well over 40. The theoretical limit of flow panels now lies not in the available fluorophores or […]

Washing Whole Blood Without a Centrifuge – Human and Mouse

Lysing and washing red blood cells using traditional microtiter plates or tubes may result in significant cell loss and damage. There is now a better way to lyse RBCs while achieving clean antibody staining and lysis as good as or better than traditional methods. The new Laminar Wash™ System offers centrifuge-less blood lysis and leukocyte […]

Flow Cytometry Survey Results – How Do You Compare to Your Peers?

Results from a December 2017 survey of 68 flow cytometry users and managers in pharma and biotech. Flow cytometrists were also asked about a new product from Curiox that washes cells without centrifugation. How do you compare to your peers? See the results for: Sample Volume Top User and Manager Concerns Unmet Needs  

Enhancing Cell Recovery and Debris Removal for Frozen Lymphocytes for Single Cell Multi-Omics

Now Watchinghttps://www.youtube.com/watch?v=aJQahE_Bf5I Description In this webinar, Dr. Bertrand Yeung and Dr. Antonino Montalbano present their experience using Laminar Wash technology for single-cell genomics and proteogenomics. BioLegend manufactures CITE-Seq assay kits (TotalSeq). A co-developer of the method and Curiox Business Development Director (formally of BioLegend), Dr. Bertrand Yeung explains how extensive cell washing is critical to study […]

Wash, Rinse, Repeat. The Benefits of Flow Cytometry Workflow Simplification

Now Watchinghttps://www.youtube.com/watch?v=rq7dyfKhygY Description Dr. Arnaud Colantonio, formally the Associate Director of analytical development at Adicet Bio (presently with Kite/Gilead) speaks to the simplified workflows, reproducibility, and easy assay transfer between departments brought about by Laminar Wash technology (at the time called DA-cell) adoption at Adicet Bio. Adicet Bio is developing an off the shelf gamma delta T-cell product, which […]

Centrifuge-less Whole Blood Staining with Laminar Wash in Biosafety Cabinet

Now Watchinghttps://www.youtube.com/watch?v=bl5M1syUb3M Description Immunophenotyping of whole blood samples using flow cytometry provides valuable pre-clinical and clinical information. Using a centrifuge to process whole blood samples compromises sample quality due to low cell retention and viability. Laminar Wash systems gently retain precious cells without a centrifuge, which is especially true for rare or sensitive immune cell […]

Centrifuge-Less Flow Cytometry – Meet the Inventor

Now Watchinghttps://www.youtube.com/watch?v=oy5VHnY0Na8 Description In this webinar, gain insights into Laminar Wash technology from the inventor himself, Dr Namyong Kim. Laminar Wash systems offer a centrifuge-less, automated, and consistent method to preparing cell samples for flow and mass cytometry, as well as single-cell ‘omics. By eliminating centrifugation, laminar flow gently washes the cells in your samples, […]