Breathe Easy with Better Models
Now Watching Research for Activating Macrophages and Dendritic Cells with Nanomedicine in Lung Carcinoma https://youtu.be/wljKOIcYsUM Macrophages can mediate phagocytosis of cancer cells and cytotoxic tumor killing, and engage in effective bidirectional interactions with components of the innate and adaptive immune system. Given the continuous recruitment of myelomonocytic cells into tumor tissues, macrophages are candidates for […]
An immunophenotype-coupled transcriptomic atlas of human hematopoietic progenitors
Zhang, X., Song, B., Carlino, M. J., Li, G., Ferchen, K., Chen, M., Thompson, E. N., Kain, B. N., Schnell, D., Thakkar, K., Kouril, M., Jin, K., Hay, S. B., Sen, S., Bernardicius, D., Ma, S., Bennett, S. N., Croteau, J., Salvatori, O., . . . Grimes, H. L. (2024). Nature Immunology, 25(4), 703–715. https://doi.org/10.1038/s41590-024-01782-4 […]
Automated Characterization Method for Validation of Epigenomic Editors in T Cells
Now Watchinghttps://youtu.be/-oRv-U2VMHg To view the transcript click here. Robust characterization methods are important analytical tools for phenotypic characterization in cell and gene therapy. For high-parameter flow cytometry methods, sample preparation is a highly complex and mostly manual procedure. Traditional flow cytometry methods are limited in throughput and resource intensive, which make them suboptimal for discovery screening […]
A novel mass cytometry protocol optimized for immunophenotyping of low-frequency antigen- specific T cells
Balz, K., Grange, M., Pegel, U., Karamya, Z. A., Mello, M., Zhou, X., Berger, T., Bloch, K., Dunham, D., Chinthrajah, S., Nadeau, K., Luche, H., & Skevaki, C. (2024b). Frontiers in Cellular and Infection Microbiology, 13. https://doi.org/10.3389/fcimb.2023.1336489 Understanding antigen-specific T-cell responses, for example, following virus infections or allergen exposure, is of high relevance for the […]
User Manual—HT2100 System
Laminar Wash HT2100 Station User Manual
The GENTIL Method for Isolation of Human Adult Cardiomyocytes from Cryopreserved Tissue for Proteomic Analyses
Waknitz, M., Luecke, L. B., Mesidor, R., Wojtkiewicz, M., Castro, C., & Gundry, R. L. (2023). Methods in Molecular Biology, 145–167. https://doi.org/10.1007/978-1-0716-3527-8_9 Heart failure is a serious clinical and economic health care problem, and its clinical progression is linked to pathological cardiac remodeling. Due to the heterogeneity of heart failure, lack of animal models to […]
Nanomedicine-based co-delivery of a calcium channel inhibitor and a small molecule targeting CD47 for lung cancer immunotherapy
Guo, Y., Bao, Q., Hu, P., & Shi, J. (2023). Nature Communications, 14(1). https://doi.org/10.1038/s41467-023-42972-2 Pro-tumoral macrophages in lung tumors present a significant challenge in immunotherapy. Here, we introduce a pH-responsive nanomedicine approach for activating anti-tumoral macrophages and dendritic cells. Using a layered double hydroxide nanosheet carrier, we co-deliver a T-type calcium channel inhibitor (TTA-Q6) and […]
Future-Proofing Single Cell Applications
Now Watchinghttps://youtu.be/wL2tsRsL9TA Technology has evolved from those early days of batching thousands of cells together to decrypt the millions of base pairs that make up genetic information. Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq), brings together surface protein phenotyping (similar to flow cytometry) and single-cell RNA sequencing (scRNA-seq). This is achieved by using […]
Getting to the Heart of the Matter: Novel Analytical Technologies Provide a New Perspective on a Complex Organ
Now Watchinghttps://www.youtube.com/watch?v=R-YT0iVN86c The heart is a 380-million-year-old organ, yet we do not have a complete understanding of the molecular landscape in the normal human adult heart. Of the many molecule classes present in the heart, we are especially lacking in our understanding of the cell surface proteins present on different cell types in the heart. […]
Critical Quality Metrics in Cell Sample Prep for Cell & Gene Therapy Bioanalytics
Now Watchinghttps://youtu.be/vEgp_FSQHF8 In this panel discussion, Virginia Litwin, Ph.D. (CRL), Christoph Eberle, MICR, FRSPH (CRL), Mahwish Natalia, MA, Ph.D. (Takeda Oncology), and Melvin Lye, MBA (Curiox Biosystems) discuss the role of partnerships between early- and late-stream analytical assay developers. While tackling unique challenges in validating flow cytometric methods for Chimeric Antigen Receptor (CAR) T cells, […]