Immunopeptidomic MHC-I Profiling and Immunogenicity Testing Identifies Tcj2 as a New Chagas Disease mRNA Vaccine Candidate
Versteeg, L., Adhikari, R., Robinson, G., Lee, J., Wei, J., Islam, N., Keegan, B., Russell, W. K., Poveda, C., Villar, M. J., Jones, K., Bottazzi, M. E., Hotez, P., Tijhaar, E., & Pollet, J. (2024). PLoS Pathogens, 20(12), e1012764. https://doi.org/10.1371/journal.ppat.1012764 Trypanosoma cruzi is a protozoan parasite that causes Chagas disease. Globally 6 to 7 million […]
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies
Sixun Chen, Tan Dai Nguyen, Kang-Zheng Lee, Dan Liu, https://doi.org/10.1016/j.biotechadv.2024.108434 Adoptive immunotherapy shows great promise as a treatment for cancer and other diseases. Recent evidence suggests that the therapeutic efficacy of these cell-based therapies can be enhanced by the enrichment of less-differentiated T cell subpopulations in the therapeutic product, giving rise to a need for […]
Profiling Circulating T cells and Tumor Landscape for Predicting Responses to PD-1 blockade in Patients with Non-Small Cell Lung Cancer
Dutta, N. (2024, September 5). https://gupea.ub.gu.se/handle/2077/81804 Immune checkpoint blockade (ICB) has significantly improved outcomes in non-small cell lung cancer (NSCLC), yet only a subset of patients achieves durable responses. This underscores the need for effective methods to assess ICB efficacy. This thesis investigates the response to PD-1 blockade in NSCLC patients across stages I to […]
Nasal tissue-resident memory CD4+ T cells persist after influenza A virus infection and provide heterosubtypic protection
NimithaR. Mathew, Romain Gailleton, Lydia Scharf, Karin Schön, Anneli Strömberg, Nils Lycke, Mats Bemark, Ka-Wei Tang, Davide Angelettihttps://doi.org/10.1101/2024.07.06.602325 CD4 tissue-resident memory T (CD4 TRM) cells are crucial adaptive immune components involved in preventing influenza A virus (IAV) infection. Despite their importance, their physiological role in the upper respiratory tract, the first site of contact with IAV, remains unclear. Here, we find that, after IAV infection, antigen-specific CD4 TRM persist in the […]
An immunophenotype-coupled transcriptomic atlas of human hematopoietic progenitors
Zhang, X., Song, B., Carlino, M. J., Li, G., Ferchen, K., Chen, M., Thompson, E. N., Kain, B. N., Schnell, D., Thakkar, K., Kouril, M., Jin, K., Hay, S. B., Sen, S., Bernardicius, D., Ma, S., Bennett, S. N., Croteau, J., Salvatori, O., . . . Grimes, H. L. (2024). Nature Immunology, 25(4), 703–715. https://doi.org/10.1038/s41590-024-01782-4 […]
Single cell view of tumor microenvironment gradients in pleural mesothelioma
Giotti, B., Dolasia, K., Zhao, W., Cai, P., Sweeney, R., Merritt, E., Kiner, E., Kim, G. S., Bhagwat, A., Nguyen, T., Hegde, S., Fitzgerald, B. G., Shroff, S., Dawson, T., Garcia-Barros, M., Abdul-Ghafar, J., Chen, R., Gnjatic, S., Soto, A., . . . Tsankov, A. M. (2024). Cancer Discovery, 14(11), 2262–2278. https://doi.org/10.1158/2159-8290.cd-23-0017 Immunotherapies have shown […]
Anti-TIGIT antibody improves PD-L1 blockade through myeloid and Treg cells
Guan, X., Hu, R., Choi, Y., Srivats, S., Nabet, B. Y., Silva, J., McGinnis, L., Hendricks, R., Nutsch, K., Banta, K. L., Duong, E., Dunkle, A., Chang, P. S., Han, C., Mittman, S., Molden, N., Daggumati, P., Connolly, W., Johnson, M., . . . Patil, N. S. (2024). Anti-TIGIT antibody improves PD-L1 blockade through myeloid […]
Immunomodulatory effects and improved outcomes with cisplatin-versus carboplatin-based chemotherapy plus atezolizumab in urothelial cancer
Galsky, M. D., Guan, X., Rishipathak, D., Rapaport, A. S., Shehata, H. M., Banchereau, R., Yuen, K., Varfolomeev, E., Hu, R., Han, C., Li, H., Liang, Y., Vucic, D., Wang, L., Zhu, J., Yu, H., Herbst, R. H., Hajaj, E., Kiner, E., . . . Mariathasan, S. (2024). Cell Reports Medicine, 5(2), 101393. https://doi.org/10.1016/j.xcrm.2024.101393 In metastatic urothelial […]
Automated Characterization Method for Validation of Epigenomic Editors in T Cells
Now Watchinghttps://youtu.be/-oRv-U2VMHg To view the transcript click here. Robust characterization methods are important analytical tools for phenotypic characterization in cell and gene therapy. For high-parameter flow cytometry methods, sample preparation is a highly complex and mostly manual procedure. Traditional flow cytometry methods are limited in throughput and resource intensive, which make them suboptimal for discovery screening […]
A novel mass cytometry protocol optimized for immunophenotyping of low-frequency antigen- specific T cells
Balz, K., Grange, M., Pegel, U., Karamya, Z. A., Mello, M., Zhou, X., Berger, T., Bloch, K., Dunham, D., Chinthrajah, S., Nadeau, K., Luche, H., & Skevaki, C. (2024b). Frontiers in Cellular and Infection Microbiology, 13. https://doi.org/10.3389/fcimb.2023.1336489 Understanding antigen-specific T-cell responses, for example, following virus infections or allergen exposure, is of high relevance for the […]