Disruption of SUV39H1-mediated H3K9 methylation sustains CAR T cell function
Jain, N., Zhao, Z., Koche, R. P., Antelope, C., Gozlan, Y., Montalbano, A., Brocks, D., Lopez, M., Dobrin, A., Shi, Y., Gunset, G., Giavridis, T., & Sadelain, M. (2023). Cancer Discovery, 14(1), 142–157. https://doi.org/10.1158/2159-8290.cd-22-1319 Suboptimal functional persistence limits the efficacy of adoptive T cell therapies. CD28-based chimeric antigen receptors (CARs) impart potent effector function to […]
Nanomedicine-based co-delivery of a calcium channel inhibitor and a small molecule targeting CD47 for lung cancer immunotherapy
Guo, Y., Bao, Q., Hu, P., & Shi, J. (2023). Nature Communications, 14(1). https://doi.org/10.1038/s41467-023-42972-2 Pro-tumoral macrophages in lung tumors present a significant challenge in immunotherapy. Here, we introduce a pH-responsive nanomedicine approach for activating anti-tumoral macrophages and dendritic cells. Using a layered double hydroxide nanosheet carrier, we co-deliver a T-type calcium channel inhibitor (TTA-Q6) and […]
Reinvigoration of Progenitor-Exhausted CD8 T Cells by anti-CTLA-4 Contributes to the Sustained Activity of Combination Checkpoint Blockade
Wang, Kevin and Coutifaris, Paulina and Brocks, David and Solis, Sabrina and Han, Nicholas and Manne, Sasikanth and Kiner, Evgeny and Sachar, Chirag and George, Sangeeth and Yan, Patrick and Kier, Melanie W. and Laughlin, Amy I. and Kothari, Shawn and Giles, Josephine R. and Mathew, Divij and Ghinnagow, Rheem and Alanio, Cecile and Flowers, […]
Critical Quality Metrics in Cell Sample Prep for Cell & Gene Therapy Bioanalytics
Now Watchinghttps://youtu.be/vEgp_FSQHF8 In this panel discussion, Virginia Litwin, Ph.D. (CRL), Christoph Eberle, MICR, FRSPH (CRL), Mahwish Natalia, MA, Ph.D. (Takeda Oncology), and Melvin Lye, MBA (Curiox Biosystems) discuss the role of partnerships between early- and late-stream analytical assay developers. While tackling unique challenges in validating flow cytometric methods for Chimeric Antigen Receptor (CAR) T cells, […]
Surfaceome mapping of primary human heart cells with CellSurfer uncovers cardiomyocyte surface protein LSMEM2 and proteome dynamics in failing hearts
Luecke, L. B., Waas, M., Littrell, J., Wojtkiewicz, M., Castro, C., Burkovetskaya, M., Schuette, E. N., Buchberger, A. R., Churko, J. M., Chalise, U., Waknitz, M., Konfrst, S., Teuben, R., Morrissette-McAlmon, J., Mahr, C., Anderson, D. R., Boheler, K. R., & Gundry, R. L. (2023). Nature Cardiovascular Research, 2(1), 76–95. https://doi.org/10.1038/s44161-022-00200-y Cardiac cell surface proteins […]
Could Debris Be Masking Your True Results
Now Watchinghttps://www.youtube.com/watch?v=TlQaihGArto Sample preparation is the stage that can make or break an assay’s performance. Preserving the natural state of samples without having to sacrifice population size can be the difference between innovation and stagnation. Now more than ever, we need dependable, reproducible results that don’t require a lab magician. With Laminar Wash, we can […]
Automating Cell Therapy Product: Constraints and Product Qualification for Fit-to-Process
Now Watchinghttps://www.youtube.com/watch?v=s-ptVJc2HpU When cells matter, it is key to prioritize their care at every stage. Clear resolution and retention of rare cell populations proves to be an issue in cell therapy processing utilizing traditional centrifugation methods. Automated, reproducible sample prep that does not rely on manual handling is the missing piece to saving precious samples, […]
How to wash away barriers to therapeutic cancer vaccine development
Developing neoantigen-based cancer vaccines necessitates comprehensive cellular characterization for iterative therapeutic improvement, which Laminar Wash™ systems may facilitate at multiple steps. Vaccines are probably the second-most effective advancement in the annals of human medicine, after sanitation. Most of us recognize the value of vaccines in infectious disease. Yet, swept under the rug amongst the COVID-19 global pandemic is another form of immune-boosting injection combating a terrible […]
Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
Now Watching Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries Elijah W. Chen*1, Chyan Ying Ke*2,3, Joanna Brzostek*1, Nicholas R. J. Gascoigne1, Vasily Rybakin1,41Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Singapore Immunology Network, A*STAR, 3Curiox Biosystems, 4Department of Immunobiology, Rega Institute for Medical Research, Katholieke Universiteit (KU) Leuven * These authors […]
Development of Centrifuge Independent Assays for Investigating T cell Signaling Pathways
Now Watchinghttps://www.youtube.com/watch?v=PtgqPNdoEeE Dr. Joanna Brzostek and Elijah Chen, of Dr. Nicholas Gascoigne’s Laboratory at National University of Singapore, describe their use of Laminar Wash technology (FKA DA-Cell) and T-cell activation, both looking at thymocytes and peripheral T-cells. In their work they encounter some very common problems, some of which are related to looking at the […]