White Paper: improved sample preparation for immuno-oncology studies
White paper: improved sample preparation for immuno-oncology studies
Scientists from Charles River Laboratories and Curiox Biosystems collaborated to characterize the performance and workflow of Laminar WashTM systems in isolating and preparing tumor infiltrating leukocytes (TILs) from anti-PD1-challenged CT26 mice, as a model system.
Compared to conventional centrifuge-based methods, Laminar Wash technology:
- Improved recovery of viable TILs from dissociated tumors
- Reduced inter-operator variation in TIL recovery
- Shortened labor-intensive hands-on washing steps
The novel Laminar Wash system represents an elegant, reproducible, and partially automated approach to recovering viable and well-defined TIL subpopulations for quantification and analysis by multicolor flow cytometry.