POSTER 2025 – Gentle Media Exchange on an Automated Platform : High Cell Retention, Imaging-Compatible Workflow

Unlock the full poster to see how C-FREE™ Pluto platform transforms media exchange for imaging and flow cytometry workflows. This study demonstrates how gentle, centrifuge-free washing and programmable low-shear media exchange preserve cell integrity, boost retention, and deliver cleaner, more reliable data. Explore side-by-side comparisons with manual methods, insights from whole blood immunophenotyping, and performance […]
The Automation Conversation: What Pharma and Flow Cores Can Learn from Each Other
WEBINAR The Automation Conversation: What Pharma and Flow Cores Can Learn from Each Other Experts from AbbVie and GLIIFCA on modernizing flow cytometry workflows with automation “We had a bunch of disparate instruments and I had to carry plates from one instrument to another…The missing link was washing.” -Ryan Duggan AbbVie Get the Recording 60 […]
POSTER 2025 – Redefining Automated Cell Washing: Centrifuge-Less C-FREE™ Pluto Wash Configurations for Reliable Retention Across Diverse Cell Types

This poster summarizes a side-by-side comparison of manual vs. centrifuge-free workflows for whole blood immunophenotyping. Using automated antibody prep, staining, lysis, and programmable wash steps, the Pluto System achieved comparable cell retention, comparable population frequencies, and improved consistency across donors—all with reduced hands-on time.Download the full poster for detailed methods, figures, and analysis. Fill in […]
Shared Principles, Consistent Results: Pluto Wash vs. Laminar Wash
By Melvin Lye, Senior Director of Scientific Affairs Introduction: Questioning a Long-Held Belief For decades, suspension cell workflows followed a rigid belief: centrifugation and pelleting were the only way to wash cells. This dogma shaped countless assays but also introduced variability, operator fatigue, and cell loss. Curiox challenged this in 2018 with Laminar Wash™ (LW), proving that […]
POSTER 2025 – Reducing Operator-Dependent Variability in Immunophenotyping Through Automated Antibody Panel Preparation

Manual antibody cocktailing in high-dimensional flow cytometry often introduces user-to-user variability and consumes valuable hands-on time. This study evaluated an automated workflow using the Curiox C-FREE™ Pluto Workstation for antibody cocktailing, benchmarking it against manual methods across 10- and 24-color panels. Results showed that: Reproducibility improved: Replicate cocktail variation consistently stayed below 5%. Data quality […]
Advancing Antibody Cocktail Solutions: Empowering CROs with Pluto Automation

By Melvin Lye, Senior Director of Scientific Affairs The preclinical CRO market is projected to reach USD 8.4 billion by 2030, driven by the rise in high-throughput studies and the demand for reproducible biomarker data in drug development. Yet, many Contract Research Organizations (CROs) still rely on manual pipetting for antibody cocktailing, creating bottlenecks that threaten […]
Automating Flow Cytometry Sample Prep: An ROI Calculation

Automating Flow Cytometry Sample Prep: An ROI Calculation Manual flow cytometry sample preparation is labor-intensive and costly, with multiple touchpoints for antibody cocktail prep, cell lysis, and staining. Each manual step adds labor cost, training time, and risk of error. Here we highlight the ROI of automated sample preparation, showing how Pluto Workstation reduces hands-on […]
Automating Antibody Cocktail Preparation for Flow Cytometry – White Paper

WHITEPAPER Automating Antibody Cocktail Preparation for Flow Cytometry This whitepaper shows how C-FREE™ Pluto Workstations (LT/MT/HT) automate antibody master mix generation with easy workflow setup and walk-away execution—delivering stain indices equivalent or better than manual prep, lower CVs on key markers, and major reductions in hands-on time—all at costs comparable to conventional pipette workstations. See […]
See How Stanford Automated Antibody Prep and Flow Cytometry Sample Staining

The Human Immune Monitoring Center used Pluto Workstation to streamline complex immunophenotyping workflows—faster, easier, and more reproducible. Download Now Automating Antibody Cocktail Preparation and Sample Staining with the C-FREE™ Pluto Workstation. Pluto Workstation – Unlock Automation in Your Lab The C-FREE™ Pluto Workstation eliminates variability and increases walkaway time in multicolor flow cytometry workflows. C-FREE™ […]
Standardizing Flow Cytometry

Thank you for Registering – Watch the Full Tutorial Below “Standardizing Flow Cytometry: Advances in Antibody Preparation and Automation for Reliable Immune Monitoring” – as presented at CYTO 2025 https://youtu.be/LRzSU3tURCA Understand the drivers of the reproducibility crisis in flow cytometry and its impact and the on clinical and research workflows. Key Takeaways: See practical solutions for […]