Tag: flow cytometry

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

Now Watching Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries  Elijah W. Chen*1, Chyan Ying Ke*2,3, Joanna Brzostek*1, Nicholas R. J. Gascoigne1, Vasily Rybakin1,41Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Singapore Immunology Network, A*STAR, 3Curiox Biosystems, 4Department of Immunobiology, Rega Institute for Medical Research, Katholieke Universiteit (KU) Leuven * These authors […]

Developing a 43 color panel on Cytek Aurora using Laminar Wash for Sample Prep

The advent of modern flow cytometers and flow cytometric methods has accelerated researchers’ ability to enhance the resolution of samples analyzed. Within a span of 20 years, flow cytometry panels have ballooned from a handful of channels to well over 40. The theoretical limit of flow panels now lies not in the available fluorophores or […]

Washing Whole Blood Without a Centrifuge – Human and Mouse

Lysing and washing red blood cells using traditional microtiter plates or tubes may result in significant cell loss and damage. There is now a better way to lyse RBCs while achieving clean antibody staining and lysis as good as or better than traditional methods. The new Laminar Wash™ System offers centrifuge-less blood lysis and leukocyte […]

Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data

Now Watchinghttps://www.youtube.com/watch?v=kAUfEX1oQKY This webinar is presented by Sue Reynolds, Ph.D. Sue has extensive flow cytometry experience working in industry as a flow core manager, application scientist, field marketing and market development manager. Watch this webinar to learn about: Impacts of centrifugation on your cell samples and data Introduction to the working principles and products of […]

Centrifuge-less Whole Blood Staining with Laminar Wash in Biosafety Cabinet

Now Watchinghttps://www.youtube.com/watch?v=bl5M1syUb3M Description Immunophenotyping of whole blood samples using flow cytometry provides valuable pre-clinical and clinical information. Using a centrifuge to process whole blood samples compromises sample quality due to low cell retention and viability. Laminar Wash systems gently retain precious cells without a centrifuge, which is especially true for rare or sensitive immune cell […]

Centrifuge-Less Flow Cytometry – Meet the Inventor

Now Watchinghttps://www.youtube.com/watch?v=oy5VHnY0Na8 Description In this webinar, gain insights into Laminar Wash technology from the inventor himself, Dr Namyong Kim. Laminar Wash systems offer a centrifuge-less, automated, and consistent method to preparing cell samples for flow and mass cytometry, as well as single-cell ‘omics. By eliminating centrifugation, laminar flow gently washes the cells in your samples, […]

Benefits of Using the Curiox Laminar Wash System in Mass Cytometry Experiments

Now Watchinghttps://www.youtube.com/watch?v=qOP26iVEnQs Description Jorgen Adolfsson is head of the Mass Cytometry Unit at Linköping University in Sweden, which is part of the SciLifeLab National Infrastructure. In this webinar Jorgen speaks about his experience using the Laminar Wash system. Jorgen describes reduction of antibody usage by ~50% with preserved signal and reproducibility. Laminar Wash technology has […]

White Paper—Handling Frozen Lymphocytes for HLA Flow Crossmatches Using Laminar Flow

Now Watchinghttps://www.youtube.com/watch?v=a-Hi4s19lQc Description Blanca Ponce-Ngo was the Transplant Immunology Lab Manager at Montefiore Medical Center in NY. Her webinar focuses on three main topics related to the handling of frozen samples for HLA Crossmatching as follows: 1) Comprehensive transfer of a Flow Crossmatching Assay 2) Impact of using frozen samples required by the National Kidney […]