Immunopeptidomic MHC-I Profiling and Immunogenicity Testing Identifies Tcj2 as a New Chagas Disease mRNA Vaccine Candidate
Versteeg, L., Adhikari, R., Robinson, G., Lee, J., Wei, J., Islam, N., Keegan, B., Russell, W. K., Poveda, C., Villar, M. J., Jones, K., Bottazzi, M. E., Hotez, P., Tijhaar, E., & Pollet, J. (2024). PLoS Pathogens, 20(12), e1012764. https://doi.org/10.1371/journal.ppat.1012764 Trypanosoma cruzi is a protozoan parasite that causes Chagas disease. Globally 6 to 7 million […]
The GENTIL Method for Isolation of Human Adult Cardiomyocytes from Cryopreserved Tissue for Proteomic Analyses
Waknitz, M., Luecke, L. B., Mesidor, R., Wojtkiewicz, M., Castro, C., & Gundry, R. L. (2023). Methods in Molecular Biology, 145–167. https://doi.org/10.1007/978-1-0716-3527-8_9 Heart failure is a serious clinical and economic health care problem, and its clinical progression is linked to pathological cardiac remodeling. Due to the heterogeneity of heart failure, lack of animal models to […]
Surfaceome mapping of primary human heart cells with CellSurfer uncovers cardiomyocyte surface protein LSMEM2 and proteome dynamics in failing hearts
Luecke, L. B., Waas, M., Littrell, J., Wojtkiewicz, M., Castro, C., Burkovetskaya, M., Schuette, E. N., Buchberger, A. R., Churko, J. M., Chalise, U., Waknitz, M., Konfrst, S., Teuben, R., Morrissette-McAlmon, J., Mahr, C., Anderson, D. R., Boheler, K. R., & Gundry, R. L. (2023). Nature Cardiovascular Research, 2(1), 76–95. https://doi.org/10.1038/s44161-022-00200-y Cardiac cell surface proteins […]
Using high throughput technologies to understand heterogeneous drug response
GE Boyle (2023), University of Washington Heterogeneity is an inherent quality of biological systems, and is one of the properties that facilitates adaptation of organisms to new environments and stimuli. However, the scientific method relies on probabilistic reasoning wherein sample measurements are assumed to represent the population as a whole. In situations where this assumption […]