Automate and standardize your B cell sample preparation workflow
How Curiox Can Help
B cells, or B lymphocytes, are a type of white blood cell that plays a crucial role in the adaptive immune system. They are primarily responsible for producing antibodies that recognize and bind to specific antigens.
B cells are central to humoral immunity, the aspect of immunity that involves the production of antibodies circulating in body fluids. They can present antigens to T cells, aiding in the activation of other immune responses. Some B cells, after an initial infection, can become memory B cells, which provide long-term immunity by quickly responding to future exposures to the same antigen.
Clinical relevance
B cells play a role in vaccines (which stimulate B cell responses for long-lasting immunity), autoimmune diseases (abnormal B cell activity can trigger the immune system to mistakenly attack the body’s own tissue), immunodeficiencies (defects in B cell development can lead to increased susceptibility to infections), and B cell lymphomas (certain cancers originate from malignant B cells).
B cell sample preparation presents distinct challenges. Subpopulations are fragile and sensitive to mechanical stress, making centrifugation-based workflows a persistent source of cell loss, viability reduction, and staining variability in B lymphocyte flow cytometry. These issues compound in high-parameter panels, where preparation inconsistency affects resolution across many markers simultaneously.
B cells are commonly analyzed using:
Centrifugation introduces mechanical stress that can reduce B cell viability, cause inconsistent pelleting, and introduce preparation artifacts — all of which affect data quality in immunophenotyping and single-cell workflows.
Curiox C-FREE™ technology enables fully automated B cell sample preparation on the Pluto Workstation — from antibody cocktailing through centrifuge-free cell washing and staining in a single session. For high-parameter B cell immunophenotyping panels, this means:
Submit the form below to request more information on centrifuge-free B cell sample preparation.
