Automated Antibody Cocktailing and Sample Preparation Preserve B-Cell Subpopulation Resolution and Staining Quality Across Donors

This poster demonstrates how the Pluto Workstation automates centrifuge-free sample preparation and antibody cocktailing for high-parameter B-cell flow cytometry immunophenotyping. Data from an independent academic core (Stanford HIMC) show automated preparation delivers B-cell subpopulation resolution and CD19/CD20 stain index equivalent to manual processing across a 24-color panel — with automated cocktailing further reducing inter-operator variability (%CV <5% for five of six markers) and hands-on time by ~30% versus manual preparation.