AUTOMATE FLOW CYTOMETRY SAMPLE PREPARATION WITH LAMINAR WASH TECHNOLOGY
Get fast, consistent results with higher cell retention, increased resolution and superior data quality.
Laminar Wash enables distinct identification of cellular populations. Samples processed by Laminar Wash show improved staining of CD20-expressing cells, yielding superior resolution of the positive and negative cell populations, compared to classical, centrifugation-based methods.
Automated cell preparation by Laminar Wash yields cleaner and clearer data for flow cytometry
Fast, reproducible results with
increased cell retention and viability leads to superior data quality
See examples of enhanced cell retention, improved reproducibility
and time savings
Publications and other useful links
UPGRADE HOW YOU PROCESS CELLS TO
GET RELIABLY CONSISTENT DATA.
Laminar Wash technology is for scientists who believe that quantitative flow cytometry is critical to cell therapy, immuno-oncology, and other fields where cell analysis is important.
Flow cytometry depends on data consistency and reproducibility to yield biologically relevant information. Yet traditional sample prep methods often fail to deliver on these key criteria. How can we address this challenge?
By moving away from centrifugation, the traditional way of processing your cells, and automating your flow cytometry workflow, from sample preparation to data analysis using laminar washing. This process allows for standardization of sample preparation methods for various cell analysis applications.
Laminar Wash technology significantly improves debris removal, increasing the retention and viability of rare cell populations. The distinct resolution and identification of target and rare cellular populations supports the gamut of therapeutic development, from biomarker identification and discovery, to predicting cellular expansion success during manufacturing.
By eliminating centrifugation and supernatant removal steps
(e.g., aspirating and flicking), Laminar Wash reduces sample handling and hands-on time, freeing your lab to take on more challenging endeavors.
If you’re interested in obtaining the most reproducible data among users and locations, then Laminar Wash technology is for you.
"Due to its underlying principle, the Laminar Wash (technology) means less mechanical stress on cells and less cell loss from a sample that is compared to conventional centrifugation steps."
Dr. Christoph Eberle, Charles River Laboratories
LAMINAR WASH TECHNOLOGY
Comparison of an immuno-staining workflow for the preparation of a single cell suspension for flow cytometry analysis. Laminar Wash technology is equally effective in washing but without the use of a centrifuge.
LAMINAR WASH IS AN AFFORDABLE AND SIMPLE SOLUTION TO AUTOMATE SAMPLE PREP FOR FLOW CYTOMETRY:
- Improved resolution of cell populations, including rare cell types
- Reduced debris and cellular aggregation
- Significant time saving of 70~90 % in processing cells, allowing scientists to spend more time on analysis and science
- Keeping cells un-stressed in their native state, improving viability
- Practical integration with cell analysis workflows, particularly in combination with automated data analysis
CENTRIFUGE-LESS RED BLOOD CELL LYSIS AND IMMUNOSTAINING WHOLE BLOOD FOR FLOW CYTOMETRY USING LAMINAR WASH™ TECHNOLOGY
LAMINAR WASH ADDS VALUE TO RESEARCH AND DEVELOPMENT
Improved enumeration of γδ T Cells with Laminar Wash™ in whole blood assay
Laminar Wash Improves Gating of Live Cells
- Better retention of live cells
- Higher stain index of VD2 marker
- Higher enumeration of rare gamma/delta T cells
Improved Operator Consistency by Automating Only the Wash Step
Laminar Wash Reduces Operator Variability.
After adopting the Laminar Wash technology users experienced low CVs in populations between operators and between experimental days.
ALL YOU NEED TO KNOW ABOUT THE LAMINAR WASH HT2000 SYSTEM
EFFORTS TO STANDARDIZE FLOW CYTOMETRY
EuroFlow consists of 20 expert academic research centers and one industry group in the fields of flow cytometry and molecular diagnostics. Their aim is to create protocols that will standardize quantitative flow cytometry for diagnostic applications. Their website presents several published protocols and other useful references, which you can access at the link below.
NIST (National Institute of Standard and Technology)
This United States government organization has a program on quantitative flow cytometry measurements that develops reference materials, methodology, and procedures enabling quantitative measurements on cells. One such reference protocol in development uses CD4 expression on healthy human T lymphocytes as a reference biomarker control.
AUTOMATE YOUR FLOW CYTOMETRY WORKFLOW