AUTOMATE FLOW CYTOMETRY SAMPLE PREPARATION WITH LAMINAR WASH TECHNOLOGY

Get fast, consistent results with higher cell retention, increased resolution and superior data quality.

Flow Cytometry Sample Preparation Dot Plot Results

Automated cell preparation by Laminar Wash yields cleaner and clearer data for flow cytometry

Fast, reproducible results with
increased cell retention and viability leads to superior data quality

See examples of enhanced cell retention, improved reproducibility
and time savings

Publications and other useful links

Overview

UPGRADE HOW YOU PROCESS CELLS TO
GET RELIABLY CONSISTENT DATA.

Laminar Wash technology is for scientists who believe that quantitative flow cytometry is critical to cell therapy, immuno-oncology, and other fields where cell analysis is important.

Flow cytometry depends on data consistency and reproducibility to yield biologically relevant information. Yet traditional sample prep methods often fail to deliver on these key criteria. How can we address this challenge?

By moving away from centrifugation, the traditional way of processing your cells, and automating your flow cytometry workflow, from sample preparation to data analysis using laminar washing. This process allows for standardization of sample preparation methods for various cell analysis applications.

Laminar Wash technology significantly improves debris removal, increasing the retention and viability of rare cell populations. The distinct resolution and identification of target and rare cellular populations supports the gamut of therapeutic development, from biomarker identification and discovery, to predicting cellular expansion success during manufacturing.

By eliminating centrifugation and supernatant removal steps
(e.g., aspirating and flicking), Laminar Wash reduces sample handling and hands-on time, freeing your lab to take on more challenging endeavors.

If you’re interested in obtaining the most reproducible data among users and locations, then Laminar Wash technology is for you.

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"Due to its underlying principle, the Laminar Wash (technology) means less mechanical stress on cells and less cell loss from a sample that is compared to conventional centrifugation steps."

Dr. Christoph Eberle, Charles River Laboratories
Flow Cytometry Sample Preparation
Technology

TRADITIONAL CENTRIFUGATION

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LAMINAR WASH TECHNOLOGY

Comparison of an immuno-staining workflow for the preparation of a single cell suspension for flow cytometry analysis. Laminar Wash technology is equally effective in washing but without the use of a centrifuge.

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LaminarWash_in_Action-1
Benefits

LAMINAR WASH IS AN AFFORDABLE AND SIMPLE SOLUTION TO AUTOMATE SAMPLE PREP FOR FLOW CYTOMETRY:

  • Improved resolution of cell populations, including rare cell types
  • Reduced debris and cellular aggregation
  • Significant time saving of 70~90 % in processing cells, allowing scientists to spend more time on analysis and science
  • Keeping cells un-stressed in their native state, improving viability
  • Practical integration with cell analysis workflows, particularly in combination with automated data analysis
Featured Resource

Overcoming challenging cell analysis with
next-generation sample prep technology

Scientific Data

LAMINAR WASH ADDS VALUE TO RESEARCH AND DEVELOPMENT

Improved enumeration of γδ T Cells with Laminar Wash™ in whole blood assay
Laminar Wash Improves Gating of Live Cells
  • Better retention of live cells
  • Higher stain index of VD2 marker
  • Higher enumeration of rare gamma/delta T cells
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Improved Operator Consistency by Automating Only the Wash Step

Laminar Wash Reduces Operator Variability.

After adopting the Laminar Wash technology users experienced low CVs in populations between operators and between experimental days.

Recent Publications & Resources View All Publications >

    Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways

    2021 | Frontiers in Immunology

    The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut

    2021 | Microbiome

    CTLA-4 blockade drives loss of Treg stability in glycolysis-low tumours

    2021 | Nature

    Peritoneal Fluid Cytokines Reveal New Insights of Endometriosis Subphenotypes

Recent Publications & Resources View All Publications >

    Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways

    2020  |  Nature Cancer

    The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut

    2020  |  Nature Cancer

    CTLA-4 blockade drives loss of Treg stability in glycolysis-low tumours

    2020  |  Nature Cancer

    Peritoneal Fluid Cytokines Reveal New Insights of Endometriosis Subphenotypes

    2020  |  Nature Cancer

EFFORTS TO STANDARDIZE FLOW CYTOMETRY

EuroFlow

EuroFlow consists of 20 expert academic research centers and one industry group in the fields of flow cytometry and molecular diagnostics. Their aim is to create protocols that will standardize quantitative flow cytometry for diagnostic applications. Their website presents several published protocols and other useful references, which you can access at the link below.

NIST (National Institute of Standard and Technology)

This United States government organization has a program on quantitative flow cytometry measurements that develops reference materials, methodology, and procedures enabling quantitative measurements on cells. One such reference protocol in development uses CD4 expression on healthy human T lymphocytes as a reference biomarker control.

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Get Started

AUTOMATE YOUR FLOW CYTOMETRY WORKFLOW

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FEATURED PRODUCTS

2
Laminar Wash HT2000

A reliable and easy-to-use benchtop instrument for high-throughput flow cytometry labs.

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Laminar Wash AUTO 1000

Complete Automation Solution for Surface Staining and Intracellular Staining for Flow Cytometry.

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Laminar Wash Mini

An affordable, benchtop instrument for low-throughput sample preparation for flow cytometry