Category: Laminar Wash

High-throughput antibody discovery to cell surface proteins

View original Presentation Nadiezda Fernandez Oropeza: Okay, good afternoon everyone. Hello, my name is Nadiezda Fernandez Oropeza, and I am the US Field Application Manager at Curiox Biosystems. I thank you for joining me today to talk about the importance of sample prep, and the effects it can have in the quality of your data. […]

High-throughput antibody discovery to cell surface proteins

Now Watchinghttps://youtu.be/LJc8mh2qNpo Advances in the field of flow cytometry, particularly in the areas of plate-based sampling and high-throughput assays have greatly enabled antibody discovery processes. A major consideration with high-throughput flow cytometry has been the time and workflow for removing non-specific antibody binding to cells or protein coated beads by washing via centrifugation. No-wash flow-based […]

The First Generation AUTO 1000 Experience – Reasons to Believe

Now Watchinghttps://www.youtube.com/watch?v=diFhoIAebLg In this talk Cecilia Kwong shares her insights on the roadblocks of automating traditional sample preparation workflows and how she developed translational metrics for assay delivery with the Laminar Wash AUTO 1000 with a focus on: Identifying automation solutions fit for process Breakdown of translational metrics used Assessment and performance of the Laminar Wash […]

Surfaceome mapping of primary human heart cells with CellSurfer uncovers cardiomyocyte surface protein LSMEM2 and proteome dynamics in failing hearts

Luecke, L. B., Waas, M., Littrell, J., Wojtkiewicz, M., Castro, C., Burkovetskaya, M., Schuette, E. N., Buchberger, A. R., Churko, J. M., Chalise, U., Waknitz, M., Konfrst, S., Teuben, R., Morrissette-McAlmon, J., Mahr, C., Anderson, D. R., Boheler, K. R., & Gundry, R. L. (2023). Nature Cardiovascular Research, 2(1), 76–95. https://doi.org/10.1038/s44161-022-00200-y Cardiac cell surface proteins […]

Overcoming challenges of Nuclei and Cell Preparation: A scientific perspective by S2 genomics and Curiox Story

Now Watchinghttps://youtu.be/inLymDAn4EI Single-cell transcriptome analysis has been extensively applied in humans and animal models to uncover gene expression heterogeneity between the different cell types of a tissue or an organ. Upstream processing may introduce inherent challenges that mask true analysis and increase user variability. Here we present a simple, yet combinatorial strategy from buffer and […]

Impact of Novel Curiox Laminar Wash on Nuclei Retention and Other Downstream Applications

Now Watchinghttps://youtu.be/U0oWMffKFlM Cell type-specific nuclei populations can be used for a wide range of downstream sequencing applications including transcriptomic and epigenomic analyses. Cerevance’s breakthrough use of Laminar Wash™ Technology in their proprietary NETSseq platform has enabled an improvement in nuclei retention as well as reagent cost reductions and time savings. This Labroots Webinar features David […]

Automation Enables Innovation

“Time is currency. Be careful how you spend it.” – Byron L. Reeder Every second spent on manual processes is a second taken away from potential revenue. Every day, scientists are racing the clock between sample preparation, running experiments, analyzing data and much more. Juggling these responsibilities along with looming deadlines, while pushing for new […]

White Paper—The Cell Analysis Conundrum, Garbage In/Garbage Out

WHITEPAPER The Cell Analysis Conundrum—Garbage In / Garbage Out Why sample preparation determines the quality of your flow cytometry and single-cell data Centrifuge-less Higher Cell Recovery Reduced Debris Standardized Workflows The whitepaper shows why centrifuge-less Laminar Wash™ is the proven solution for high-quality cell sample preparation. C-FREE™ Pluto Wash builds on the same principle—gentle, controlled […]