C-FREE™ Pluto Code – Replace Centrifugation with Pipette-Based Automation
Home / Replace Centrifugation with Pipette-Based Automation Many cell workflows still rely on repeated spin–decant–resuspend steps. C-FREE™ Pluto Code introduces an alternative: pipette-based automation that enables wash-and-mix sequences, without relying on centrifugation. Install the Pluto Code script library into your existing liquid handler for full walkaway workflows, no hardware upgrades required. Request Info C-FREE™ Pluto […]
Validated 384-Well C-FREE™ Washing for High-Throughput Cell Prep
Validated 384-Well Centrifuge-Free Washing for High-Throughput Cell Prep Technical and biological validation of centrifuge-free washing at 384-well scale using the C-FREE™ Pluto platform. Enabling high-throughput cell prep automation Moving to 384-well formats increases throughput and reduces reagent use—but washing at this scale introduces real risks: sample loss, uneven retention, and assay variability. This application note […]
From Gentle Wash to Harmonized Workflows: How the Field Scaled Next
By Melvin Lye, Curiox Biosystems For much of the history of flow cytometry, centrifugation has been treated as an accepted compromise. It is efficient, familiar, and deeply embedded in laboratory practice. At the same time, its limitations (cell loss, mechanical stress, and operator-dependent variability) have long been recognized, even if rarely interrogated explicitly in published work. As […]
POSTER 2026 – Pluto Code – Enabled Centrifuge-Free Automation: Toward a Universal Standard for Gentle Wash
This poster presents Pluto Code, a software-defined framework that enables centrifuge-free, gentle cell washing directly on standard liquid-handling platforms. Using C-FREE™ wash physics implemented as executable code, Pluto Code delivers reproducible wash and mix performance across diverse cell-based assays without additional hardware. Validation data demonstrate comparable or improved cell retention, debris removal, and assay consistency […]
Harmonization in Cell Preparation: Preserving Data Comparability Across Sites, CROs, and Automation Platforms
By Mallory Griffin, Curiox Biosystems The problem: comparable data starts upstream Cell-based assays have benefited from major advances in instrumentation, reagents, and analysis software. Measurement technologies and data analysis are increasingly automated, standardized, and shared across organizations. Cell preparation, however, remains one of the least controlled parts of the workflow. Even when the same protocol […]
POSTER 2025 – Gentle Media Exchange on an Automated Platform : High Cell Retention, Imaging-Compatible Workflow
Unlock the full poster to see how C-FREE™ Pluto platform transforms media exchange for imaging and flow cytometry workflows. This study demonstrates how gentle, centrifuge-free washing and programmable low-shear media exchange preserve cell integrity, boost retention, and deliver cleaner, more reliable data. Explore side-by-side comparisons with manual methods, insights from whole blood immunophenotyping, and performance […]
The Automation Conversation: What Pharma and Flow Cores Can Learn from Each Other
WEBINAR The Automation Conversation: What Pharma and Flow Cores Can Learn from Each Other Experts from AbbVie and GLIIFCA on modernizing flow cytometry workflows with automation “We had a bunch of disparate instruments and I had to carry plates from one instrument to another…The missing link was washing.” -Ryan Duggan AbbVie Get the Recording 60 […]
POSTER 2025 – Redefining Automated Cell Washing: Centrifuge-Less C-FREE™ Pluto Wash Configurations for Reliable Retention Across Diverse Cell Types
This poster summarizes a side-by-side comparison of manual vs. centrifuge-free workflows for whole blood immunophenotyping. Using automated antibody prep, staining, lysis, and programmable wash steps, the Pluto System achieved comparable cell retention, comparable population frequencies, and improved consistency across donors—all with reduced hands-on time.Download the full poster for detailed methods, figures, and analysis. Fill in […]
Shared Principles, Consistent Results: Pluto Wash vs. Laminar Wash
By Melvin Lye, Senior Director of Scientific Affairs Introduction: Questioning a Long-Held Belief For decades, suspension cell workflows followed a rigid belief: centrifugation and pelleting were the only way to wash cells. This dogma shaped countless assays but also introduced variability, operator fatigue, and cell loss. Curiox challenged this in 2018 with Laminar Wash™ (LW), proving that […]
POSTER – Reducing Operator-Dependent Variability in Immunophenotyping Through Automated Antibody Panel Preparation
Manual antibody cocktailing in high-dimensional flow cytometry often introduces user-to-user variability and consumes valuable hands-on time. This study evaluated an automated workflow using the Curiox C-FREE™ Pluto Workstation for antibody cocktailing, benchmarking it against manual methods across 10- and 24-color panels. Results showed that: Reproducibility improved: Replicate cocktail variation consistently stayed below 5%. Data quality […]