POSTER 2026 – Centrifuge-Free Preparation Delivers Comparable Staining Performance for Complex Multicolor Panels: A Study from Stanford HIMC and Curiox
SCIENTIFIC POSTER Centrifuge-Free Preparation Delivers Comparable Staining Performance for Complex Multicolor Panels: A Study from Stanford HIMC and Curiox This poster presents a head-to-head comparison of automated versus manual antibody cocktail preparation for high-dimensional flow cytometry. Using the Curiox C-FREE™ Pluto Workstation, we evaluated 10- and 24-color immunophenotyping panels in collaboration with the Stanford Human […]
POSTER 2026 – Automated Centrifuge-Free PBMC Washing Flow Cytometry
SCIENTIFIC POSTER Standardizing PBMC Washing in Multi-User Flow Cytometry Labs Using Pluto ALPHA Automated Centrifuge-Free Workflows Manual centrifugation-based PBMC washing introduces operator variability, inconsistent cell recovery, and workflow bottlenecks — especially in multi-user lab settings. This poster presents a head-to-head comparison of conventional centrifugation and automated centrifuge-free washing using the Pluto ALPHA workstation across surface […]
POSTER 2026 – Automated C-FREE Workflow for Reproducible Memory T-cell and NK-cell Immunophenotyping
SCIENTIFIC POSTER Automated C-FREE™ Workflow for Reproducible Memory T-cell and NK-cell Immunophenotyping Manual staining and wash steps introduce variability in memory T-cell and NK-cell immunophenotyping. This poster evaluates whether automated antibody cocktailing and C-FREE™ sample processing on the Pluto Workstation improve reproducibility — with head-to-head data across stain index, cell retention, and population frequencies. Download […]
Holden Maecker on Automating Flow Cytometry Sample Prep
Holden Maecker, PhD to Present at Labroots Laboratory Automation Virtual Event Series 2026 Flow cytometry sample preparation is still largely manual in most labs. At the Labroots Laboratory Automation Virtual Event Series 2026 (March 18, 2026, free and virtual), Holden Maecker, PhD of Stanford’s Human Immune Monitoring Center presents a benchtop approach to changing that. […]
C-FREE™ Pluto Code – Replace Centrifugation with Pipette-Based Automation
Home / Replace Centrifugation with Pipette-Based Automation Many cell workflows still rely on repeated spin–decant–resuspend steps. C-FREE™ Pluto Code introduces an alternative: pipette-based automation that enables wash-and-mix sequences, without relying on centrifugation. Install the Pluto Code script library into your existing liquid handler for full walkaway workflows, no hardware upgrades required. Request Info C-FREE™ Pluto […]
Validated 384-Well C-FREE™ Washing for High-Throughput Cell Prep
Validated 384-Well Centrifuge-Free Washing for High-Throughput Cell Prep Technical and biological validation of centrifuge-free washing at 384-well scale using the C-FREE™ Pluto platform. Enabling high-throughput cell prep automation Moving to 384-well formats increases throughput and reduces reagent use—but washing at this scale introduces real risks: sample loss, uneven retention, and assay variability. This application note […]
From Gentle Wash to Harmonized Workflows: How the Field Scaled Next
By Melvin Lye, Curiox Biosystems For much of the history of flow cytometry, centrifugation has been treated as an accepted compromise. It is efficient, familiar, and deeply embedded in laboratory practice. At the same time, its limitations (cell loss, mechanical stress, and operator-dependent variability) have long been recognized, even if rarely interrogated explicitly in published work. As […]
POSTER 2026 – Pluto Code – Enabled Centrifuge-Free Automation: Toward a Universal Standard for Gentle Wash
SCIENTIFIC POSTER This poster presents Pluto Code, a software-defined framework that enables centrifuge-free, gentle cell washing directly on standard liquid-handling platforms. Using C-FREE™ wash physics implemented as executable code, Pluto Code delivers reproducible wash and mix performance across diverse cell-based assays without additional hardware. Validation data demonstrate comparable or improved cell retention, debris removal, and […]
Harmonization in Cell Preparation: Preserving Data Comparability Across Sites, CROs, and Automation Platforms
By Mallory Griffin, Curiox Biosystems The problem: comparable data starts upstream Cell-based assays have benefited from major advances in instrumentation, reagents, and analysis software. Measurement technologies and data analysis are increasingly automated, standardized, and shared across organizations. Cell preparation, however, remains one of the least controlled parts of the workflow. Even when the same protocol […]
POSTER 2025 – Gentle Media Exchange on an Automated Platform : High Cell Retention, Imaging-Compatible Workflow
Unlock the full poster to see how C-FREE™ Pluto platform transforms media exchange for imaging and flow cytometry workflows. This study demonstrates how gentle, centrifuge-free washing and programmable low-shear media exchange preserve cell integrity, boost retention, and deliver cleaner, more reliable data. Explore side-by-side comparisons with manual methods, insights from whole blood immunophenotyping, and performance […]