Nasal tissue-resident memory CD4+ T cells persist after influenza A virus infection and provide heterosubtypic protection

NimithaR. Mathew, Romain Gailleton, Lydia Scharf, Karin Schön, Anneli Strömberg, Nils Lycke, Mats Bemark, Ka-Wei Tang, Davide Angelettihttps://doi.org/10.1101/2024.07.06.602325 CD4 tissue-resident memory T (CD4 TRM) cells are crucial adaptive immune components involved in preventing influenza A virus (IAV) infection. Despite their importance, their physiological role in the upper respiratory tract, the first site of contact with IAV, remains unclear. Here, we find that, after IAV infection, antigen-specific CD4 TRM persist in the […]

A unified model-based framework for doublet or multiplet detection in single-cell multiomics data

Hu, H., Wang, X., Feng, S., Xu, Z., Liu, J., Heidrich-O’Hare, E., Chen, Y., Yue, M., Zeng, L., Rong, Z., Chen, T., Billiar, T., Ding, Y., Huang, H., Duerr, R. H., & Chen, W. (2024). Nature Communications, 15(1). https://doi.org/10.1038/s41467-024-49448-x Droplet-based single-cell sequencing techniques rely on the fundamentalassumption that each droplet encapsulates a single cell, enabling […]

An immunophenotype-coupled transcriptomic atlas of human hematopoietic progenitors

Zhang, X., Song, B., Carlino, M. J., Li, G., Ferchen, K., Chen, M., Thompson, E. N., Kain, B. N., Schnell, D., Thakkar, K., Kouril, M., Jin, K., Hay, S. B., Sen, S., Bernardicius, D., Ma, S., Bennett, S. N., Croteau, J., Salvatori, O., . . . Grimes, H. L. (2024). Nature Immunology, 25(4), 703–715. https://doi.org/10.1038/s41590-024-01782-4 […]

A novel mass cytometry protocol optimized for immunophenotyping of low-frequency antigen- specific T cells

Balz, K., Grange, M., Pegel, U., Karamya, Z. A., Mello, M., Zhou, X., Berger, T., Bloch, K., Dunham, D., Chinthrajah, S., Nadeau, K., Luche, H., & Skevaki, C. (2024b). Frontiers in Cellular and Infection Microbiology, 13. https://doi.org/10.3389/fcimb.2023.1336489 Understanding antigen-specific T-cell responses, for example, following virus infections or allergen exposure, is of high relevance for the […]

Nanomedicine-based co-delivery of a calcium channel inhibitor and a small molecule targeting CD47 for lung cancer immunotherapy

Guo, Y., Bao, Q., Hu, P., & Shi, J. (2023). Nature Communications, 14(1). https://doi.org/10.1038/s41467-023-42972-2 Pro-tumoral macrophages in lung tumors present a significant challenge in immunotherapy. Here, we introduce a pH-responsive nanomedicine approach for activating anti-tumoral macrophages and dendritic cells. Using a layered double hydroxide nanosheet carrier, we co-deliver a T-type calcium channel inhibitor (TTA-Q6) and […]

Future-Proofing Single Cell Applications

Now Watchinghttps://youtu.be/wL2tsRsL9TA Technology has evolved from those early days of batching thousands of cells together to decrypt the millions of base pairs that make up genetic information. Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq), brings together surface protein phenotyping (similar to flow cytometry) and single-cell RNA sequencing (scRNA-seq). This is achieved by using […]

High-throughput workflow leveraging spectral flow cytometry and CITE-seq to characterize human bone marrow across race and gender

Song, B., Zhang, X., Bennett, S., Ma, S., Ferchen, K., Salomonis, N., & Grimes, H. L. (2023). The Journal of Immunology, 210(1_Supplement), 250.13. https://doi.org/10.4049/jimmunol.210.supp.250.13 Characterizing human bone marrow is challenging due to heterogeneity of cell types, and the rarity of some populations. To address this, we developed a workflow that bridges CITE seq and flow […]

Surfaceome mapping of primary human heart cells with CellSurfer uncovers cardiomyocyte surface protein LSMEM2 and proteome dynamics in failing hearts

Luecke, L. B., Waas, M., Littrell, J., Wojtkiewicz, M., Castro, C., Burkovetskaya, M., Schuette, E. N., Buchberger, A. R., Churko, J. M., Chalise, U., Waknitz, M., Konfrst, S., Teuben, R., Morrissette-McAlmon, J., Mahr, C., Anderson, D. R., Boheler, K. R., & Gundry, R. L. (2023). Nature Cardiovascular Research, 2(1), 76–95. https://doi.org/10.1038/s44161-022-00200-y Cardiac cell surface proteins […]

Using high throughput technologies to understand heterogeneous drug response

GE Boyle (2023), University of Washington Heterogeneity is an inherent quality of biological systems, and is one of the properties that facilitates adaptation of organisms to new environments and stimuli. However, the scientific method relies on probabilistic reasoning wherein sample measurements are assumed to represent the population as a whole. In situations where this assumption […]