WHITEPAPER

Automating Antibody Cocktail Preparation for Flow Cytometry

This whitepaper shows how C-FREE™ Pluto Workstations (LT/MT/HT) automate antibody master mix generation with easy workflow setup and walk-away execution—delivering stain indices equivalent or better than manual prep, lower CVs on key markers, and major reductions in hands-on time—all at costs comparable to conventional pipette workstations.

C-FREE™ Pluto Workstations automate antibody cocktail preparation, staining, and washing—delivering:

Up to 70% less hands-on time
<5% variability across batches
Ready-to-run in under a day—no coding required
Affordable—priced like a pipette workstation

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10–12 min read • Immediate access

What You'll Learn

End-to-end automated workflow

How Pluto automates antibody master mix prep with precise dispensing, mixing, aliquoting, and optional FMO/SCC outputs—guided by validated CSV scripts.

Ab Prep Wizard & validation

Panel definition, duplicate/expiry checks, volume margin checks, and reporting. Learn the required filename rule (CSV = script name) and rapid upload to the tablet.

Throughput & configurations

Compare LT (≤32 wells), MT (≤72), HT (≤96; 4-channel vari-span). Review time savings (~15 min hands-on) and reagent tracking that cuts waste.

Trusted by leading research centers for complex, high-dimensional immunophenotyping.

Evaluations at Stanford’s Human Immune Monitoring Center and internal Curiox studies reported equivalent or improved stain indices vs manual prep and reduced CV% across markers, with substantial reductions in hands-on time.