Validated 384-Well Centrifuge-Free Washing for High-Throughput Cell Prep

384-well application note for centrifuge-free cell washing

Technical and biological validation of centrifuge-free washing at 384-well scale using the C-FREE™ Pluto platform.

Enabling high-throughput cell prep automation

Moving to 384-well formats increases throughput and reduces reagent use—but washing at this scale introduces real risks: sample loss, uneven retention, and assay variability. This application note evaluates C-FREE™ Pluto Wash’s ability to support reliable, reproducible washing at 384-well density, using a structured validation strategy that links fluidic performance to biologically relevant endpoints.

*384-Well throughput is expected to be available in the second half of 2026 for Pluto Code and Pluto Workstation.

Application Note

12-15 min read • Immediate access

What This Study Evaluates

This application note presents a stepwise validation of C-FREE™ Pluto Wash at the 384-well format, benchmarked against established workflows:

  • Manual centrifuge washing (baseline SOP)
  • Automated Pluto washing at 96-well scale
  • Automated Pluto washing at 384-well scale

Performance is assessed using both technical and biological models, allowing separation of fluidics from biological variability.

Intended Use Cases

This application note is relevant for teams working on:

  • High-throughput staining workflows
  • Automation-ready sample preparation without centrifugation
  • Early evaluation of 384-well feasibility prior to full workflow deployment

Ready to Take the Next Step?

Want to see how Pluto could fit into your workflow? Contact our team for a personalized consultation.

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