Curiox Laminar Wash HT2000 System

Achieve higher throughputs and cleaner data with our 96-well laminar washing system

With the Curiox Laminar Wash HT2000 System, you get all the benefits of gentle laminar cell washing in a format compatible with high-throughput and automated workflows.

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Curiox Laminar Wash

Intuitive Touch-screen Interface

Quickly customize starting volume, flow rate and number of washes.

Automation-friendly Firmware & PCB

Easily integrate with automated robotic platforms

High-throughput 96-well Format

Process multiple samples at once with our gentle laminar washing technology.

Optional Buffer Exchange (BEX)

Use the touch-screen interface to choose from up to 8 different solutions.

What the HT2000 delivers:
  • Better data quality and better cell recovery than centrifugation
  • Greater reproducibility from study-to-study and operator-to-operator
  • A higher stain index for cleaner resolution of populations
  • Less cell clumping and the subsequent clogging of fluidic systems
  • Faster workflows that can completely wash 96 samples in just 3 minutes
  • Better biosafety through reduced aerosolization and a footprint that fits into most biosafety cabinets

Technology Overview

A quicker, simpler path to better data

With the HT2000’s laminar washing technology, cells are deposited onto a 96-well laminar wash plate, where a gentle flow of buffer washes away unwanted debris, such as unbound antibody or dye. Unlike with centrifugation, very little stress is placed upon the cells, ensuring the integrity of cell surface markers. The thoroughness of the laminar washing process produces cleaner, better segregated data than centrifugation-based wash methods and the gentleness of the technique enables high cell retention and viability.

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With the Curiox Laminar Wash™ we retain more cells with much less data variation between samples than our centrifuge process.

Dr Jorgen Adolfsson, Linkoping University

Traditional Workflow

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Laminar Flow Technology

Compared to a traditional, centrifuge-driven cell washing workflow, laminar cell washing is simpler and faster. With the HT2000, you can get complete washing of 96 samples in just 3 minutes.

How our plate and nozzle design washes your cells

The power of Curiox’s laminar cell washing technology lies in the unique design of the 96-sample plate. Each plate contains an array of hydrophilic spots that suspension cells can rest on via gravity. The material surrounding each spot is highly hydrophobic, acting as an impenetrable wall that separates your samples.

Upon entry into the instrument, two nozzles descend onto each side of every spot, one to dispense wash buffer and the other to aspirate the buffer and debris away. 

Each spot can wash as many as 10 million cells down to a single cell without the mechanical stress and cell losses associated with centrifugation.

See how dye is washed away from green fluorescent polystyrene beads during 2 minutes of 7 aspiration and dispense wash cycles. At the end of the cycle, the dye is gone but the polystyrene beads remain in the spot.

Scientific data

Laminar washing delivers better cell retention and cleaner, clearer data

When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.

Curiox Laminar Wash Scatter Plot Data

Laminar wash advances with neutrophils and monocytes retention for improved differential blood count

With 100ul of human whole blood, a comparison study using a 16-color flow cytometry panel focused on T cells was conducted by Laminar Washing. Whole blood lysed with ammonium chloride buffer was first centrifuged once to reduce volume, and then aliquoted to incubate with antibodies and washed on either Laminar Wash or centrifuge in polystyrene tubes. There is clear resolution of rare T cell populations such as MAIT, gamma-delta T cells and regulatory T cells with replacement of centrifuge with Laminar Wash in one wash step. The elevated retention for neutrophils and monocytes was observed and quantified via direct acquisition unto a BD Fortessa, allowing minimal cell perturbations.

Figure 2. Human red blood cells (RBCs) lysed with (NH4Cl) and washed using HT2000 Laminar Flow technology (top) show better cell retention and tighter cell populations than RBCs lysed and washed using centrifugation on samples in tubes (bottom), whether enumerated by CD45 staining or FSC-SSC scattering.

Technical Specifications
Voltage Requirements 100-200V
Power Consumption 24V/3.0A
HT2000 Dimensions 513 mm H x 262 mm W x 302 mm D 20.2 in H x 10.3 in W x 11.9 in D
Weight 14kg (31lbs)
Accessories and Components
Laminar Wash™ 96-well plate (with a regular lid) 96-DC-CL-05
Laminar Wash™ 96-well plate (with a regular lid) *Customised 96-DC-CL-05-CP
HT Buffer Exchanger 5 DC-BX-01-05
HT Buffer Exchanger 10 DC-BX-01-10
FEATURED RESOURCES

All you need to know about the Laminar Wash HT2000 System

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