Longitudinal single-cell analysis of SARS-CoV-2-reactive B cells uncovers persistence of early-formed, antigen-specific clones
When the body encounters a pathogen, such as a virus, the immune system develops an adaptive immune response through B-cell and T-cell responses to clear the infection and develop memory…
Read MorePP4 inhibition sensitizes ovarian cancer to NK cell-mediated cytotoxicity via STAT1 activation and inflammatory signaling
Cancerous tumors grow and prosper in an inflammatory environment at the intersection of many signaling pathways in the immune system. Since tumors exist in such a complex environment, researchers have…
Read MoreSurfaceome mapping of primary human heart cells with CellSurfer uncovers cardiomyocyte surface protein LSMEM2 and proteome dynamics in failing hearts
Cell surface proteins (surfaceome) play a significant role in the development of novel target therapies. Therefore, a comprehensive surfaceome profiling approach is necessary to identify and quantify specific markers for…
Read MoreComprehensive Benchmarking of CITE-seq versus DOGMA-seq Single Cell Multimodal Omics
While single-cell multiomics techniques represent a growing dictionary for generating enormous insight into cell biology, objective assessments of utility, strengths, and weaknesses are lacking. Researchers at the the University of…
Read MoreSevere COVID-19 infection is associated with aberrant cytokine production by infected lung epithelial cells rather than by systemic immune dysfunction
Researchers at the University of Chicago used their Laminar Wash HT2000 system to safely prepare peripheral immune cells from COVID-19 patients with moderate and severe disease for flow cytometry in…
Read MoreInsights into adult atopic dermatitis heterogeneity derived from circulating biomarker profiling in patients with moderate-to-severe disease
Scientists at Eli Lilly were characterizing circulatory inflammatory profiles and underlying systemic disease heterogeneity within atopic dermatitis (AD) patients. The multiplexing study of several pro-inflammatory mediators distinguished between baseline disease…
Read MoreLarge scale cytokine profiling uncovers elevated IL12‑p70 and IL‑17A in severe pediatric acute respiratory distress syndrome
Investigators in Singapore utilized Curiox’s DropArray (LT210) multiplex immunoassay system to profile 45 cytokines, chemokines, and growth factors in children with severe and non-severe PARDS. This revealed a differential immunological…
Read MorePrimary Human Dendritic Cells and Whole-Blood Based Assays to Evaluate Immuno-Modulatory Properties of Heat-Killed Commensal Bacteria
Norton, J.E., Jr.; Kommineni, S.; Akrivoulis, P.; Gutierrez, D.A.; Hazuda, D.J.; Swaminathan, G. Primary Human Dendritic Cells and Whole-Blood Based Assays to Evaluate Immuno-Modulatory Properties of Heat-Killed Commensal Bacteria. Vaccines…
Read MoreSingle-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways
Stewart A., Ng JC-F, Wallis G, et al. Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways. Front. Immunol. 2021;12:602539.
Read MoreThe pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut
Arnold JW, Roach J, Fabela S, et al. The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut. Microbiome. 2021;9(1):31.
Read More