Tag: flow cytometry

Towards standardizing flow cytometry sample preparation protocols without centrifugation

Now Watchinghttps://youtu.be/NTTfiQiqhqE In this sponsored webinar by the flowcytometryUK society webinar, renowned flow cytometrist Derek Davies, Ph.D. of the Francis Crick Institute hosted a discussion featuring Curiox Applications Scientist Noma Ngwenya and Charles River Laboratories Principal Scientist III and Flow Cytometry Core Director Christoph Eberle, Ph.D.  They explored how Laminar Wash™ technology enhances sample preparation for flow cytometry by […]

A Missing Sample Prep Tool for Single-Cell Biology is Found

Gentle and semi-automated Laminar Wash™ technology removes background, debris, doublets, and dead time, extending single-cell ‘omics budgets. Single-cell ‘omics technologies are achieving a new paradigm in biology. As Eadweard Muybridge applied photography to settle the “unsupported transit” controversy surrounding the 19th century galloping horse, immunologists are utilizing single-cell transcriptomics (scRNA) to tease apart the heterogeneity […]

How to wash away barriers to therapeutic cancer vaccine development

Developing neoantigen-based cancer vaccines necessitates comprehensive cellular characterization for iterative therapeutic improvement, which Laminar Wash™ systems may facilitate at multiple steps. Vaccines are probably the second-most effective advancement in the annals of human medicine, after sanitation. Most of us recognize the value of vaccines in infectious disease. Yet, swept under the rug amongst the COVID-19 global pandemic is another form of immune-boosting injection combating a terrible […]

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

Now Watching Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries  Elijah W. Chen*1, Chyan Ying Ke*2,3, Joanna Brzostek*1, Nicholas R. J. Gascoigne1, Vasily Rybakin1,41Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Singapore Immunology Network, A*STAR, 3Curiox Biosystems, 4Department of Immunobiology, Rega Institute for Medical Research, Katholieke Universiteit (KU) Leuven * These authors […]

Developing a 43 color panel on Cytek Aurora using Laminar Wash for Sample Prep

The advent of modern flow cytometers and flow cytometric methods has accelerated researchers’ ability to enhance the resolution of samples analyzed. Within a span of 20 years, flow cytometry panels have ballooned from a handful of channels to well over 40. The theoretical limit of flow panels now lies not in the available fluorophores or […]

Washing Whole Blood Without a Centrifuge – Human and Mouse

Lysing and washing red blood cells using traditional microtiter plates or tubes may result in significant cell loss and damage. There is now a better way to lyse RBCs while achieving clean antibody staining and lysis as good as or better than traditional methods. The new Laminar Wash™ System offers centrifuge-less blood lysis and leukocyte […]