Category: Laminar Wash

Washing Whole Blood Without a Centrifuge – Human and Mouse

Lysing and washing red blood cells using traditional microtiter plates or tubes may result in significant cell loss and damage. There is now a better way to lyse RBCs while achieving clean antibody staining and lysis as good as or better than traditional methods. The new Laminar Wash™ System offers centrifuge-less blood lysis and leukocyte […]

Single Cell Genomics Workflow with Laminar Wash (LW) in Biosafety Cabinet

Now Watchinghttps://www.youtube.com/watch?v=3bMAZ9cP75g Description Single cell genomics provide valuable information on the profiles and differences between individual cells from a heterogenous cell population, such as immune profiling of tumor microenvironments. Preparation of single cell suspensions for single cell genomic applications is crucial to obtaining high quality sequencing data. Factors such as poor cell retention and contamination […]

Preparation of Anti-Evaporation Lid

Now Watchinghttps://www.youtube.com/watch?v=hXtr0qFrqe4 Description This video describes how to set up the anti-evaporation lid for a DropArray plate used in a miniaturized multiplex bead immunoassay. In order to keep the small volume droplet from evaporating, a humidified environment is created by using the anti-evaporation lid.

Laminar Wash MINI1000 Setup and Workflow in Biosafety Cabinet

Now Watchinghttps://www.youtube.com/watch?v=wbZIFLzIwEc Description This video offers a detailed guide to using and maintaining the Laminar Wash MINI 1000 System for flow cytometry sample preparation. As demonstrated by our scientist in the video, setting up of the Laminar Wash MINI 1000 is straightforward, including pre-programmed priming steps. The Laminar Wash MINI 1000 System can be easily […]

Improved Cell Recovery by Skipping Centrifugation

Now Watchinghttps://www.youtube.com/watch?v=NJearwAfTwc Description Laminar Wash technology offers a centrifuge-less sample preparation method, which gently and continuously washes the cells in your samples. By eliminating centrifugation, sample preparation no longer subjects the cells to mechanical stress. This leads to better cell retention and produces higher quality data. In the first part of the webinar, watch how […]

Enhancing Cell Recovery and Debris Removal for Frozen Lymphocytes for Single Cell Multi-Omics

Now Watchinghttps://www.youtube.com/watch?v=aJQahE_Bf5I Description In this webinar, Dr. Bertrand Yeung and Dr. Antonino Montalbano present their experience using Laminar Wash technology for single-cell genomics and proteogenomics. BioLegend manufactures CITE-Seq assay kits (TotalSeq). A co-developer of the method and Curiox Business Development Director (formally of BioLegend), Dr. Bertrand Yeung explains how extensive cell washing is critical to study […]

Wash, Rinse, Repeat. The Benefits of Flow Cytometry Workflow Simplification

Now Watchinghttps://www.youtube.com/watch?v=rq7dyfKhygY Description Dr. Arnaud Colantonio, formally the Associate Director of analytical development at Adicet Bio (presently with Kite/Gilead) speaks to the simplified workflows, reproducibility, and easy assay transfer between departments brought about by Laminar Wash technology (at the time called DA-cell) adoption at Adicet Bio. Adicet Bio is developing an off the shelf gamma delta T-cell product, which […]

Centrifuge-less Whole Blood Staining with Laminar Wash in Biosafety Cabinet

Now Watchinghttps://www.youtube.com/watch?v=bl5M1syUb3M Description Immunophenotyping of whole blood samples using flow cytometry provides valuable pre-clinical and clinical information. Using a centrifuge to process whole blood samples compromises sample quality due to low cell retention and viability. Laminar Wash systems gently retain precious cells without a centrifuge, which is especially true for rare or sensitive immune cell […]

Centrifuge-Less Flow Cytometry – Meet the Inventor

Now Watchinghttps://www.youtube.com/watch?v=oy5VHnY0Na8 Description In this webinar, gain insights into Laminar Wash technology from the inventor himself, Dr Namyong Kim. Laminar Wash systems offer a centrifuge-less, automated, and consistent method to preparing cell samples for flow and mass cytometry, as well as single-cell ‘omics. By eliminating centrifugation, laminar flow gently washes the cells in your samples, […]