Improve your mass cytometry data by reducing background and enhancing cell retention.
Laminar Wash can help you achieve less data variation between samples by gentle washing
Reduce debris and enhance cell retention in mass cytometry sample preparation
See examples of reduced variation and more intact singlets compared to centrifugation
Links to useful resources related to mass cytometry
CHANGE THE WAY YOU PROCESS CELLS TO GET RELIABLY CONSISTENT DATA.
Mass cytometry is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than labeled with fluorescent antibodies. Since detection is by time-of-flight mass spectrometry, there is no spectral overlap of signals, which allows for higher numbers of target proteins to be analyzed.
Mass cytometry allows deep studies of both surface markers and intracellular cytokines. It is a well-documented method with many publications. Most often you will find a mass cytometry in an institution’s core facility, so preparing your samples in your own lab is key to getting good results.
“The one thing which I didn't expect it here is that you reduce usage of antibody with roughly 50% and we've preserved signal and reproducibility.”
Jorgen Adolfsson, Linkoping University
Laminar Flow Technology
Mass cytometry protocols can be quite harsh on the cells and Laminar Wash technology treats them gently as compared to centrifugation, which can result in more singlets.
BENEFITS OF LAMINAR WASH FOR MASS CYTOMETRY
Mass cytometry experiments are expensive and time consuming. Make sure all of your samples produce good data with Laminar Wash technology.
BENEFITS OF USING THE CURIOX LAMINAR WASH™ SYSTEM IN MASS CYTOMETRY EXPERIMENTS
DATA USING THE LAMINAR WASH SYSTEMS
Generate More Intact Singlets Compared to Centrifugation
A 25 isotope-labelled panel was used on PBMC samples starting with one million cells. Samples were then prepared using either Laminar Wash technology or centrifugation.
The frequency of singlets is higher in the Laminar Wash prepared samples. The number of intact cells was also significantly greater with the Laminar Wash treated samples.
Increased Reproducibility Compared to Centrifugation
In this t-SNE plot, the cells cluster depending upon the expression of all 25 markers. The Laminar Wash technology prepared replicates appear to be homogenous, meaning that the samples are indistinguishable from each other when clustered. The cluster of cells prepared using centrifugation shows three very unique samples within the cluster, meaning a cell at one end of the cluster is very different from one on the other end of the cluster.