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Enhancing Cell Recovery and Debris Removal for Frozen Lymphocytes for Single Cell Multi-Omics


In this webinar Bertrand Yeung of BioLegend and Dr. Antonino Montalbano of ImmunAI present their experience using Laminar Wash technology for single cell genomics and proteogenomics.
As the manufacturer of CITE-SEQ assay kits (TotalSEQ by BioLegend), BioLegend’s speaker Bertrand Yeung, explains one of the critical elements of the assay, cell washing. Because, if you don’t wash well enough, your background can be quite high, as well as your signal, for any given antibody in the pool.
Also, another thing that BioLegend has had a lot of request for in terms of guidance is how to stain very low cell numbers. Because, you can imagine, with very low cell numbers of anywhere between 50 and 100,000 cells, many people are worried about losing their cells during the washing.
BioLegend has found that there’s a very noticeable improvement in speed versus centrifugation in terms of processing time. It takes less than 15 minutes for us to use the Curiox for a very large number of samples, whereas it could take anywhere from 30 to 45 minutes for, maybe, 10 to 20 samples, or even much longer for many more samples. So this is very good for labs that have high throughput or need it for high throughput sample processing.
Antonino Montalbano explains that at ImmunAI when they use Laminar Wash system they have a nice recovery and the Laminar Wash system has been great for samples that have a very low number of cells. The take home message for Laminar Wash is that it really helps retain high number of cells. And, it helps also with multiplexing different samples together.

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